CD16 – RMab

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Ihc Of Cd16 On An Ffpe Bone Marrow Tissue

IHC of CD16 on a FFPE Bone Marrow Tissue

Specification Sheets
Safety Data Sheet
Intended UseFor In Vitro Diagnostic Use
Summary and Explanation

CD16 is a low affinity Fc receptor, found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies which then activates NK cells for antibody-dependent cell-mediated cytotoxicity. A lack of CD16 in a given population of neutrophils may indicate prematurity, as could be caused by a left shift due to neutrophilic leukocytosis induced by tissue necrosis or bacterial infection.

The IHC of CD16 is useful in the differential diagnosis of hepatosplenic gamma delta T-cell lymphoma and gamma delta T-cell large granular lymphocyte leukemia from other peripheral T-cell lymphomas, such as mucosal and cutaneous gamma delta T-cell lymphoma. A significant decrease can be seen in the number of granulocytes expressing CD16 in chronic myelomonocytic leukemia compared to chronic myelogenous leukemia and control bone marrow biopsy, probably related to dysgranulopoiesis. It has also been demonstrated that colorectal carcinoma patients with high CD16+ cell infiltration is associated with improved overall survival after adjusting for known prognostic factors and this association was independent from CD8+ lymphocyte infiltration and presence of metastases.

Antibody TypeRabbit MonoclonalCloneEP364
IsotypeIgGReactivityParaffin, Frozen
LocalizationCytoplasmic, MembranousControlPlacenta, Liver, Breast, Spleen, Thymus, Lung
PresentationCD16 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Catalog No.Antibody TypeDilutionVolume/QTY
BSB 3321PredilutedReady-To-Use3.0 ml
BSB 3322PredilutedReady-To-Use7.0 ml
BSB 3323PredilutedReady-To-Use15.0 ml
BSB 3324Concentrated1:50-1:2000.1 ml
BSB 3325Concentrated1:50-1:2000.5 ml
BSB 3326Concentrated1:50-1:2001.0 ml
BSB 3327Control Slides5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.

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