IHC of NSE on an FFPE Pancreas Tissue
|For In Vitro Diagnostic Use
|Summary and Explanation
|Neuron-Specific Enolase (NSE, Enolase 2) is a human gene. It makes a phosphopyruvate hydratase. This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. NSE is present in high concentration in neurons and in central and peripheral neuroendocrine cells; therefore, NSE reacts with cells of neural and neuroendocrine lineage. If neoplastic cells coexpress keratins and NSE, neuroendocrine differentiation is probable. However, neural tumors that do not express keratin, and show no staining with NSE, would not exclude neural or neuroendocrine differentiation. Thus, detection of neural and neuroendocrine lineage requires the use of panels which include NSE and other markers such as keratin, chromogranin, synaptophysin and neurofilament.
|Pancreas, Brain, Pituitary, Adrenal, Thyroid
|NSE is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
|Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.