IHC of Cytokeratin 5 & 14 on an FFPE Breast Tissue Tissue
|Intended Use||For In Vitro Diagnostic Use|
|Summary and Explanation||Cytokeratin 5 is a type II cytokeratin found in squamous cell epithelium, myoepithelial cells of the breast and the basal cells of the prostate. Cytokeratin 14 is a Type I polypeptide found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Together, they form the cytoskeleton of epithelial cells.
Cytokeratin 5 is expressed in most Epithelial Mesotheliomas but not by most Pulmonary Adenocarcinomas and can be used to differentiate between the two. Cytokeratin 14 antibody labels the basal layer of stratifying squamous and non-squamous epithelia and recognizes Basal Cell Carcinomas and Squamous Cell Carcinomas. Anti-CK14 has been demonstrated to be useful in differentiating Squamous Cell Carcinomas from other epithelial tumors. This antibody has also been useful in separating oncocytic tumors of the kidney from renal mimics, as well as in determining metaplastic Carcinomas of the Breast. Anti- CK5, along with Anti-CK14, has found application in identifying the basaloid phenotype of breast carcinoma, a tumor with poor prognosis.
|Antibody Type||Rabbit Monoclonal||Clone||EP24/EP61|
|Localization||Cytoplasmic||Control||Breast, Prostate, Skin, Prostatic carcinoma|
|Presentation||CK 5 & 14 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.|
|Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.|