IHC of CD74 on an FFPE Tonsil Tissue
|For In Vitro Diagnostic Use
|Summary and Explanation
CD74, also known as the MHC Class II-associated invariant chain (II), is a Type II transmembrane protein which binds to the peptide-binding groove of newly-synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen-presenting cells such as B-lymphocytes (from before the pre-B-cell stage to before the plasma-cell stage), macrophages and monocytes, together with many epithelial cells. CD74 stains predominantly germinal-center lymphocytes and B-cell lymphomas but rarely T-cell lymphomas. It stains the cell membrane but a paranuclear globular labeling is also noted. CD74 is useful in differentiating Atypical Fibroxanthoma from Malignant Fibrous Histiocytoma, as well as Small-cell Lung Carcinoma from Non-small cell Lung Carcinomas.
|Tonsil, Lymph Node
|CD74 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
|Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.