BOB.1 – RMab

SKU: 65c7c6414469 Categories: , ,


IHC of BOB.1 on an FFPE Tonsil Tissue

Specification Sheets
Safety Data Sheet
Intended UseFor In Vitro Diagnostic Use
Summary and ExplanationThe BOB.1 protein is a co-activator that interacts with OCT-1 and/or OCT-2 transcription factors, and is critical in germinal center formation and immunoglobulin production. The strongest expression of BOB.1 is found in the germinal center, mantle-zone B cells, and plasma cells. Because BOB.1/OBF.1 are germinal center derived, L&H cells in Nodular Lymphocyte Predominant Hodgkin’s Lymphoma are consistently immunoreactive for BOB.1. Conversely, the Hodgkin/Reed-Sternberg cells in classical Hodgkin’s Lymphoma either do not express both or express only one of the two proteins or neither.
In Diffuse Large B-cell Lymphomas, the highest expression levels for BOB.1/OBF.1 are reported in Follicular Center Lymphomas, Diffuse Large B-cell Lymphomas, and Burkitt Lymphomas. B-CLL, MALT-type, and Mantle Cell Lymphomas score negative or display a heterogenous/weaker activity. The strong nuclear expression of BOB.1 and Oct-2 by Germinal Center Derived Lymphomas makes these antibodies a novel class of broad spectrum B-lineage immunohistochemical markers in the differential diagnosis of Lymphomas, specifically between Primary Mediastinal B-cell Lymphoma from classical Hodgkin’s Disease .
Antibody TypeRabbit MonoclonalCloneEP114
IsotypeIgGReactivityParaffin, Frozen
LocalizationCytoplasmic, NuclearControlTonsil, Lymph Node
PresentationBOB.1 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Catalog No.Antibody TypeDilutionVolume/QTY
BSB 6380PredilutedReady-To-Use3.0 ml
BSB 6381PredilutedReady-To-Use7.0 ml
BSB 6382PredilutedReady-To-Use15.0 ml
BSB 6383Concentrated1:100-1:5000.1 ml
BSB 6384Concentrated1:100-1:5000.5 ml
BSB 6385Concentrated1:100-1:5001.0 ml
BSB 6386Control Slides5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.

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