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ATRX – MMab

SKU: 942a19b941ab Categories: , ,

IHC of ATRX on a FFPE Astrocytoma Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation

ATRX is also known as ATP-dependent helicase ATRX, X-linked helicase II, or X-linked nuclear protein (XNP) is a protein that in humans is encoded by the ATRX gene. Mutations of the ATRX gene are associated with an X-linked mental retardation (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome.

Mutation/loss of ATRX expression has been described in anaplastic gliomas. A study explored the role of ATRX status in the molecular classification of anaplastic gliomas and its impact on survival. Loss of ATRX expression was detected in 45 % of anaplastic astrocytomas (AA), 27 % of anaplastic oligoastrocytomas (AOA) and 10 % of anaplastic oligodendrogliomas (AO). Survival analysis showed a marked separation of IDH mutant astrocytic tumors into two groups based on ATRX status: tumors with ATRX loss had a significantly better prognosis.

Another study conducted an immunohistochemical analysis of ATRX expression in adult diffuse gliomas and found ATRX immunoreactivity of tumor cells was either almost totally absent or completely retained in all cases. There was perfect concordance between the IHC results and ATRX mutation status. ATRX loss was observed in 54.5, 30.8 and 0.0% of grades II/III astrocytomas, oligoastrocytomas and oligodendrogliomas, respectively, and 12.7% of glioblastomas. Another recent study analyzed the use of ATRX, IDH and 1p/19q codeletion in a series astrocytomas, oligodendrogliomas, oligoastrocytomas and glioblastomas and presented an algorithm based on stepwise analysis with initial immunohistochemistry for ATRX and IDH1-R132H followed by 1p/19q analysis then by IDH sequencing, which reduces the number of molecular analyses and which has a far better association with patient outcome.

Antibody Type Mouse Monoclonal Clone BSB-108
Isotype IgG2a/K Reactivity Paraffin, Frozen
Localization Nuclear Control Breast, Tonsil, Testis, Salivary Gland, Placenta, TCC, Astrocytoma
Presentation ATRX is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 3293 Prediluted Ready-To-Use 3.0 ml
BSB 3294 Prediluted Ready-To-Use 7.0 ml
BSB 3295 Prediluted Ready-To-Use 15.0 ml
BSB 3296 Concentrated 1:50-1:200 0.1 ml
BSB 3297 Concentrated 1:50-1:200 0.5 ml
BSB 3298 Concentrated 1:50-1:200 1.0 ml
BSB 3299 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.

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