IHC of BOB.1 on an FFPE Tonsil Tissue
| Intended Use | For In Vitro Diagnostic Use | |||||||||||||||||||||||||||||||||||
| Summary and Explanation | The BOB.1 protein is a co-activator that interacts with OCT-1 and/or OCT-2 transcription factors, and is critical in germinal center formation and immunoglobulin production. The strongest expression of BOB.1 is found in the germinal center, mantle-zone B cells, and plasma cells. Because BOB.1/OBF.1 are germinal center derived, L&H cells in Nodular Lymphocyte Predominant Hodgkin’s Lymphoma are consistently immunoreactive for BOB.1. Conversely, the Hodgkin/Reed-Sternberg cells in classical Hodgkin’s Lymphoma either do not express both or express only one of the two proteins or neither. In Diffuse Large B-cell Lymphomas, the highest expression levels for BOB.1/OBF.1 are reported in Follicular Center Lymphomas, Diffuse Large B-cell Lymphomas, and Burkitt Lymphomas. B-CLL, MALT-type, and Mantle Cell Lymphomas score negative or display a heterogenous/weaker activity. The strong nuclear expression of BOB.1 and Oct-2 by Germinal Center Derived Lymphomas makes these antibodies a novel class of broad spectrum B-lineage immunohistochemical markers in the differential diagnosis of Lymphomas, specifically between Primary Mediastinal B-cell Lymphoma from classical Hodgkin’s Disease . | |||||||||||||||||||||||||||||||||||
| Antibody Type | Rabbit Monoclonal | Clone | EP114 | |||||||||||||||||||||||||||||||||
| Isotype | IgG | Reactivity | Paraffin, Frozen | |||||||||||||||||||||||||||||||||
| Localization | Cytoplasmic, Nuclear | Control | Tonsil, Lymph Node | |||||||||||||||||||||||||||||||||
| Presentation | BOB.1 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative. | |||||||||||||||||||||||||||||||||||
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| Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product. | ||||||||||||||||||||||||||||||||||||
