Topoisomerase II alpha – RMab

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Ihc Of Topo Iia On An Ffpe Hsil Of The Cervix

IHC of Topo IIa on an FFPE HSIL of the Cervix

Specification Sheets
Safety Data Sheet
Intended UseFor In Vitro Diagnostic Use
Summary and ExplanationDNA Topoisomerase II alpha (Topo IIa) is a nucleic enzyme that affects the topological structure of DNA by interacting with the double-helix DNA, thus playing an important role in DNA replication, transcription, recombination, condensation, and segregation. Type II topoisomerases cut both strands of the DNA helix simultaneously in order to change the linking number of the molecule. Topo IIa is essential in the separation of daughter strands at the end of replication. Failure to separate these strands leads to cell death. In cancers, the Topo IIa is highly expressed in highly-proliferating cells.Topo IIa has been identified by DNA microarray and transcriptional profiling as a gene that is overexpressed in Cervical Carcinomas. The TOP2A gene is approximately 30 kb in size and encodes a 170 kDa protein.Topo IIa protein is expressed in proliferating cells and in numerous human malignant tumors, including colon, gastric and breast cancers, Lymphomas and others. In certain cancers, such as Peripheral Nerve Sheath Tumors, high expression of this protein is also associated with poor patient survival. Type II topoisomerases are the targets for anticancer drugs, such as topoisomerase II inhibitor therapies like the anthracyclines (Doxorubicin and Epirubicin).
Antibody TypeRabbit MonoclonalCloneRBT-Topo 2a
IsotypeIgGReactivityParaffin, Frozen
LocalizationNuclearControlTestis, Skin, Colon, Fallopian Tube, Tonsil, Lymph Node, Spleen, HSIL Cervical Cancer, Breast Cancer, Bladder TCC
PresentationTopo IIa is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Catalog No.Antibody TypeDilutionVolume/QTY
BSB 6338PredilutedReady-To-Use3.0 ml
BSB 6339PredilutedReady-To-Use7.0 ml
BSB 6340PredilutedReady-To-Use15.0 ml
BSB 6341Concentrated1:50-1:2000.1 ml
BSB 6342Concentrated1:50-1:2000.5 ml
BSB 6343Concentrated1:50-1:2001.0 ml
BSB 6344Control Slides5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.

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