BRAF V600E – RMab

3. Ihc Of Braf V600E On A Ffpe Papillary Thyroid Carcinoma Tissue Webpage

IHC of BRAF V600E on an FFPE Papillary Thyroid Carcinoma Tissue

United States
Research Use
Safety Data Sheet
Control Slides
Intended UseFor Analyte Specific Reagent
Summary and Explanation

BRAF is a human gene that makes a protein called B-Raf, which is more formally known as serine/threonine-protein kinase B-Raf.  The B-Raf protein is involved in sending signals inside cells, which are involved in directing cell growth. In 2002, it was shown to be mutated in some human cancers.  Mutations in the BRAF gene can cause disease in two ways. First, mutations can be inherited and cause birth defects. Second, mutations can appear later in life and cause cancer, as an oncogene.

Mutations in this gene have been found in cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, papillary thyroid carcinoma, non-small-cell lung carcinoma, and adenocarcinoma of the lung. The frequency of BRAF mutations varies widely in human cancers, from more than 80% in melanomas and nevi, to as little as 0–18% in other tumors, such as 1–3% in lung cancers and 5% in colorectal cancer. In 90% of the cases, thymine is substituted with adenine at nucleotide 1799. This leads to valine (V) being substituted for by glutamate (E) at codon 600 (referred to as V600E) in the activation segment that has been found in human cancers. This mutation has been widely observed in papillary thyroid carcinoma, colorectal cancer, melanoma and non-small-cell lung cancer. BRAF-V600E mutation are present in 57% of Langerhans cell histiocytosis patients. The V600E mutation is a likely driver mutation in 100% of cases of hairy cell leukemia. High frequency of BRAF V600E mutations have been detected in ameloblastoma, a benign but locally infiltrative odontogenic neoplasm.

Antibody TypeRabbit MonoclonalCloneRM8
IsotypeIgGReactivityParaffin, Frozen
LocalizationCytoplasmicControlBRAF 600E Mutated Melanoma, Papillary Thyroid Cancer
PresentationBRAF V600E is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Catalog No.Antibody TypeDilutionVolume/QTY
BSB 2824PredilutedReady-To-Use3.0 ml
BSB 2825PredilutedReady-To-Use7.0 ml
BSB 2826PredilutedReady-To-Use15.0 ml
BSB 2827Concentrated1:50-1:2000.1 ml
BSB 2828Concentrated1:50-1:2000.5 ml
BSB 2829Concentrated1:50-1:2001.0 ml
BSB 2830Control Slides5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.

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