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IHC of PSMA on an FFPE Prostate Adenocarcinoma Tissue

IHC of PSMA on an FFPE Prostate Adenocarcinoma Tissue

Specification Sheets
Safety Data Sheet


Intended Use For In Vitro Diagnostic Use
Summary and Explanation PSMA, prostate specific membrane antigen, is a Type 2 integral membrane glycoprotein found in prostate and a few other tissues. Three functionally-distinct proteins are encoded, including folylpoly-gamma-glutamate carboxypeptidase in the intestine, N-acetylated alpha-linked acidic dipeptidase 1 in the brain and prostatespecific membrane antigen in the prostate. A mutation in the intestinal form may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. The form expressed in the brain may be involved in a number of pathological conditions associated with glutamate cytotoxicity. The prostate form is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants. Although PSMA expression is highest in the prostate, detectable levels of protein are also found in the small intestine and the brain. PSMA is expressed in prostate cancer cells as a noncovalently associated homodimer. Using a secreted form of the protein, it has been demonstrated that the extracellular domain is sufficient for dimerization and that dimerization is required for enzymatic activity. When used as an immunogen, dimeric (but not monomeric) PSMA is capable of efficiently eliciting antibodies that recognize PSMA-expressing tumor cells. It is a possible therapeutic target for prostate cancer and it is being used (with radioactive antibodies) to image prostate tissue.
Antibody Type Rabbit Monoclonal Clone EP192
Isotype IgG Reactivity Paraffin, Frozen
Localization Cytoplasmic Control Prostate, Prostatic Adenocarcinoma
Presentation PSMA is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed,filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Catalog No. Antibody Type Dilution Volume/QTY
BSB 6345 Prediluted Ready-To-Use 3.0 ml
BSB 6346 Prediluted Ready-To-Use 7.0 ml
BSB 6347 Prediluted Ready-To-Use 15.0 ml
BSB 6348 Concentrated 1:50-1:200 0.1 ml
BSB 6349 Concentrated 1:50-1:200 0.5 ml
BSB 6350 Concentrated 1:50-1:200 1.0 ml
BSB 6351 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
* The PSMA antibody, clone EP192, has been manufactured using Epitomics RabMab® technology covered under Patent No.’s 5,675,063 and 7,402,409.

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