PolyDetector HRP Green Kit

Cd35 Kidney Hrp Green

IHC of CD35 on FFPE Kidney Tissue (HRP Green)

Ckoscar Colon Hrpgreen

IHC of CK OSCAR on FFPE Colon Tissue (HRP Green)


IHC of EpCAM/Ber-EP4 on FFPE Melanoma Tissue (HRP Green)
Intended UseFor In Vitro Diagnostic Use
Summary and ExplanationPolyDetector HRP Green Substrate-Chromogen Kit is suitable for use with peroxidase detection systems, which allow the demonstration of cell antigens or nucleic acids in paraffin-embedded tissues, cryostat sections, cytosmears, and cell preparations. The substrate-chromogen is the final step in the detection portion; it enables the antibody-antigen or nucleic acid complex to be viewed under a light microscope. This occurs because HRP Green acts as an electron donor in the presence of the enzyme horseradish peroxidase; HRP Green gets oxidized and produces a vibrant green color at the site of the target antigen or nucleic acid. This substrate-chromogen is particularly useful in tissues where there is endogenous melanin or with multiplex IHC or CISH.

HRP Green forms a permanent record of the results of the stain but it is partially soluble in organic solvents like alcohol or xylenes and therefore needs to be mounted with a fast alcohol/xylene protocol or using the Bio SB ChromoProtector (BSB 0151 – BSB 0156), then an organic Permanent Mounting medium such as XyGreenPermaMounter (BSB 0169-0174), PermaMounter (BSB 0094-0097) or similar permanent mounting media. HRP Green is not suitable for aqueous mounting.

PresentationThe PolyDetector HRP Green Substrate-Chromogen Kit contains a liquid HRP Green Chromogen dissolved in polymers and an HRP Green Buffer Substrate containing a buffer, hydrogen peroxide as a substrate, and a non-azide anti-microbial.
BSB 0128PolyDetector HRP Green Kit15.0 ml
BSB 0129PolyDetector HRP Green Kit50.0 ml
BSB 0130PolyDetector HRP Green Kit100.0 ml
BSB 0131PolyDetector HRP Green Kit200.0 ml
BSB 0132PolyDetector HRP Green Kit500.0 ml
BSB 0133PolyDetector HRP Green Kit1000.0 ml

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