IHC of CK Oscar on FFPE Kidney Tissue (ALK Red)
IHC of CK 34BE12 on FFPE Breast (ALK Red)
IHC of CK OSCAR on FFPE Skin Tissue (ALK Red)
|For In Vitro Diagnostic Use
|Summary and Explanation
|PolyDetector Alk Red Ready-to-Use Substrate-Chromogen is a solution suitable for use in immunohistochemical, immunocytochemical and in situ hybridization procedures using Alkaline Phosphatase Detection Systems. PolyDetector Alk Red Ready-to-Use Substrate-Chromogen allows for the demonstration of tissue antigens or nucleic acids in paraffin-embedded tissues, cryostat sections, cytosmears, and cell preparations.
The substrate-chromogen is the final step in the detection portion; it enables the antibody-antigen-enzyme complex (or nucleic-acid-probe-enzyme complex) to be viewed under a light microscope. This occurs when Alk Red, in the presence of Alkaline Phosphatase, gets deposited at the site of the target antigen or nucleic acid, producing a red color. Alk Red is soluble in organic solvents and therefore should be mounted with water-based mounting media, such as Aqua Mounter (BSB 0090-BSB 0093) or can be mounted with permanent medium when using the Bio SB ChromoProtector (BSB 0151 – BSB 0156).
|PolyDetector Alk Red Ready-to-Use Substrate-Chromogen (color forming molecule) is a ready-to-use solution that develops into a red precipitate.