Products

NKX2.2 – RMab

SKU: ccc8538aa3c2 Categories: , ,

IHC of NKX2.2 on an FFPE Pancreas Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation

Homeobox protein NKX2.2 is a protein encoded by the NKX2-2 gene. NKX2.2 is a homeodomain-containing transcription factor that plays a critical role in neuroendocrine/glial differentiation. NKX2.2 is expressed in the developing forebrain and spinal cord. Functionally, the transcription factor is thought to be involved with neuronal developing, patterning, and fate specification of neurons and oligodendrocytes.

NKX2.2 was recently reported as a valuable marker for Ewing’s sarcoma. The vast majority of Ewing’s sarcomas (85%) harbor a chromosomal translocation, most commonly t(11;22)(q24;q12) encoding an aberrant EWS-FLI transcription factor. NKX2.2 expression is tightly correlated with EWS-FLI expression, a critical downstream target that is required for the cancerous behavior of Ewing’s sarcoma. While CD99 is the classical marker for Ewing’s sarcoma diagnosis, it is relatively nonspecific. In addition to Ewing’s sarcoma, CD99 is also expressed on lymphocytes, hematopoietic cells, endothelial cells and a variety of tumors. In contrast, NKX2.2 labels 93% of Ewing’s sarcoma and only a small subset (14/130) of non-Ewing tumors, demonstrating a sensitivity of 93% and specificity of 89%. Staining with NKX2.2 can aid in the differential diagnosis of small round cell tumors.

Antibody Type Rabbit Monoclonal Clone EP336
Isotype IgG Reactivity Paraffin, Frozen
Localization Nuclear Control Pancreas, Brain, Pituitary, Colon, Ewing’s Sarcoma
Presentation NKX2.2 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 3106 Prediluted Ready-To-Use 3.0 ml
BSB 3107 Prediluted Ready-To-Use 7.0 ml
BSB 3108 Prediluted Ready-To-Use 15.0 ml
BSB 3109 Concentrated 1:50-1:200 0.1 ml
BSB 3110 Concentrated 1:50-1:200 0.5 ml
BSB 3111 Concentrated 1:50-1:200 1.0 ml
BSB 3112 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.

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